Actopaxin/Parvin Phospho(pS8)(Parva)antibody
Cat.#: 2607-1
Rabbit Monoclonal Antibody
Clone ID: EPR2174
Swiss Prot: Q9EPC1
Mol Weight: 42kDa
Size: 100ul
Description
Actopaxin (also named Parvin, Parva) is a member of the alpha actinin superfamily containing a single alpha-actinin-like actin-binding domain. The Parvin family plays a role in cell adhesion and cytoskeleton organization. Analysis of Actopaxin and its para- and orthologues suggest, that the parvins represent a new family of alpha-actinin-related proteins that mediate cell-matrix adhesion (1). Actopaxin is a widely expressed protein that binds directly to both F-actin and paxillin LD1 and LD4 motifs. It exhibits robust focal adhesion localization in several cultured cell types, but it is not found along the length of the associated actin-rich stress fibers. Actopaxin colocalizes with paxillin to rudimentary focal complexes at the leading edge of migrating cells. Results suggest an important role for actopaxin in integrin-dependent remodeling of the actin cytoskeleton during cell motility and cell adhesion (2).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:2500 - 5000
ICC: 1:100 - 250
Species Reactivity
Human, Mouse, Rat
Cross reactivity determined by western blot only.
Products Data
A. Western blot analysis on A549 cell lysates using anti- Phospho-Actopaxin (pS8) RabMAb (cat. #2607-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with LP
B. Immunofluorescent staining of HeLa cells using Phospho-specific anti-Actopaxin RabMAb (cat. # 2607-1).
Specificity
A phospho specific peptide corresponding to residues surrounding serine 8 of mouse Parvin was used as an immunogen. This antibody detects Actopaxin phosphorylated on serine 8.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
Parva antibody, 2010012A22Rik antibody, 5430400F08Rik antibody, AI225929 antibody, AU042898 antibody, Actp antibody, CH-ILKBP antibody, Parvin antibody, Alpha-parvin antibody, Actopaxin antibody
Description References
1. Olski TM et al. J Cell Sci 114(Pt.3):525-38, 2001
2. Nikolopoulos SN et al. J Cell Biol 151(7) :1435-48, 2000
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
公司地址
上海市松江区陆家圈路168弄113号
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