4E-BP1 Phospho(pT37)(EIF4EBP1)antibody

4E-BP1 Phospho(pT37)(EIF4EBP1)antibody
Cat.#: 2411-1
Rabbit Monoclonal Antibody

Clone ID: EPR729(2)Y
Swiss Prot: Q13541
Mol Weight: 17kDa
Size: 100ul

Description
4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eIF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP1. Non-phosphorylated 4E-BP1 will bind strongly to eIF4E while, the phosphorylated form will no (2). Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 70 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3). It has been reported previously that phosphorylation of 4E-BP1 on Thr 37 and Thr 46 is relatively insensitive to serum deprivation and rapamycin treatment, and that phosphorylation of these residues is required for the subsequent phosphorylation of a set of unidentified serum-responsive sites (4)

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:1000 - 2000

Species Reactivity
Human, Mouse

Cross reactivity determined by western blot only.

Products Data

Western blot analysis on 293T cell lysates using anti-Phospho-4E-BP1 (pT37) RabMAb (cat. #2411-1). Cells were either (A) untreated (B) treated with insulin.

Specificity
A phospho specific peptide corresponding to residues surrounding threonine 37 was used as an immunogen. This antibody detects 4E-BP1 phosphorylated at threonine 37.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
EIF4EBP1 antibody, 4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, MGC4316 antibody, PHAS-I antibody, Eukaryotic translation initiation factor 4E-binding protein 1 antibody, Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody

Description References
1. Pause, A., et al. 1994. Nature 371: 762-767
2. Gingras, A.-C., et al. Genes & Dev. 12: 502-513, 1998
3. Iritani, BM et al. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13180