ACHR-beta(extracellular)(CHRNB1)antibody

ACHR-beta(extracellular)(CHRNB1)antibody
Cat.#: 2123-1
Rabbit Monoclonal Antibody
Clone ID: EP2067Y
Swiss Prot: P11230
Mol Weight: 56kDa
Size: 100ul

Description
The nicotinic acetylcholine receptor controls electrical signaling between nerve and muscle cells by opening and closing a gated, membrane-spanning pore. The gate is a constricting hydrophobic girdle at the middle of the lipid bi-layer, formed by weak interactions between neighboring inner helices. When acetylcholine enters the ligand-binding domain, it triggers rotations of the protein chains on opposite sides of the entrance to the pore (1). The widespread distribution of the beta subunit suggests it may serve as a common subunit in different AChR combinations in various brain regions. Regulation of the expression of beta subunit appears to be relatively unrestrained, with an apparent excess of protein synthesized in the cytoplasm relative to that which ultimately arrives at functional targets in the plasma membrane (2).

Recommended Applications
WB, ICC, FC

Applications and Recommended Dilution Factors
WB: 1:1000 - 2000
ICC: 1:100
FC: 1:30

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on human brain lysate using anti-ACHR-beta RabMAb (cat. # 2123-1), dilution 1:1000.

Specificity
A synthetic peptide corresponding to residues in the extracellular region of human Acetylcholine receptor subunit beta was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
CHRNB1 antibody, ACHRB antibody, CHRNB antibody, CMS1D antibody, CMS2A antibody, FLJ57107 antibody, SCCMS antibody, Acetylcholine receptor subunit beta antibody, antibody

Description References
1. Miyazawa A, et al. Nature 423(6943):949-55, 2003
2. Hill JA, et al. J Neurosci 13: 1551-68, 1993