ACADM antibody

ACADM antibody
Cat.#: 2833-1
Rabbit Monoclonal Antibody

Clone ID: EPR3708
Swiss Prot: P11310
Mol Weight: 43kDa
Size: 100ul

DescriptionMedium-chain specific acyl-CoA dehydrogenase (ACADM) is a homotetramer enzyme of the mitochondrial flavoenzyme family that catalyzes the initial reaction in medium chain fatty acid beta-oxidation (1, 2). ACADM is specific for acyl chain lengths of 4 to 16 (3). It utilizes the electron transfer flavoprotein (ETF) as an electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (3). Defects in the ACADM gene cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD), a metabolic disorder characterized by fasting hypoglycemia (3). Mutation analysis can diagnose MCADD, which is currently screened in newborns, since its monogene may be modified by genetic variations (4).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:10,000-50,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data  

Western blot analysis on (A) human heart, (B) fetal liver, (C) HeLa, (D) HepG2, and (E) K562 cell lysates using anti-ACADM RabMAb (cat. # 2833-1).
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ACADM RabMAb (cat. #2833-1).
Immunofluorescent staining of HeLa cells using anti-ACADM RabMAb (cat. #2833-1).

Specificity
A synthetic peptide corresponding to residues in human ACADM was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACADM antibody, ACAD1 antibody, FLJ18227 antibody, FLJ93013 antibody, FLJ99884 antibody, MCAD antibody, MCADH antibody, Medium-chain specific acyl-CoA dehydrogenase, mitochondrial antibody, antibody

Description References
1. Zhang et al. Biochemistry 31:81-89, 1992. 2. Battaile et al. Elsevier Science B.V., 1998. 3. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 37:D169-D174(2009). 4. Gregersen et al. Hum Mutat.18(3):169-89, 2001.