高端生物型原子力显微镜AR

Controlled Delivery of DNA Origami

Due to its capacity for programmable self-assembly, well- established modes of chemical synthesis, and exceptional stability, DNA serves as a powerful nanoscale structural material.[1,2] In particular, the recent invention ofDNAorigami technology[3] has established a paradigm in which DNA’s capacity for deterministic self-assembly into essentially any discrete two-dimensional (2D) shape can be exploited for the construction of molecular ‘‘bread boards’’. For example, previous groups have demonstrated the delivery of nanopar- ticles to specific positions within an origami scaffold with nanometer-scale precision[4] and the weaving of DNA aptamers[5,6] into origami for the assembly of protein arrays.[7] However, in order to fully harness the potential of DNA as a universal nanoscale template, it is important not only to control the position of cargo material within the origami scaffold but also to accurately control the position and orientation of the

高分辨率肥大细胞细胞表面膜

Atomicforcemicroscopy(AFM)enableshigh-resolutionthree-dimensional(3D)imagingofcultured bone marrow-derivedmastcells.Cellswereimmobilizedbyaquickcentrifugationandfixationto preserve theirtransientcellularmorphologiesfollowedbyAFMcharacterizationinbuffer.This‘‘fix-and- look’’ approachpreservesthestructuralintegrityofindividualcells.Well-knownmembrane morphologies,suchasridgesandmicrovilli,arevisualized,consistentwithpriorelectronmicroscopy observations. Additionalinformationincludingthe3Dmeasurementsofthesecharacteristicfeatures are attainedfromAFMtopographs.Filopodiaandlamellopodia,associatedwithcellspreading,were capturedandvisualizedinthreedimensions.Newmorphologiesarealsorevealed,suchashigh-density ridges andmicro-craters.Thisinvestigationdemonstratesthatthe‘‘fix-and-look’’approachfollowedby AFM imagingprovidesaneffectivemeanstocharacterizethemembranestructureofhydratedcells with highresolution.Thequantitativeimagingandmeasurementspavethewayforsystematic correlation ofmembranestructuralfeatureswiththebiologicalstatusofindividualcells

花粉微结构研究

We isolated lap3-1 and lap3-2 mutants in a screen for pollen that displays abnormal stigma binding. Unlike wild-type pollen, lap3-1 and lap3-2 pollen exine is thinner, weaker, and is missing some connections between their roof-like tectum structures. We describe the mapping and identification of LAP3 as a novel gene that contains a repetitive motif found in b-propeller enzymes. Insertion mutations in LAP3 lead to male sterility. To investigate possible roles for LAP3 in pollen development, we assayed the metabolite profile of anther tissues containing developing pollen grains and found that the lap3-2 defect leads to a broad range of metabolic changes. The largest changes were seen in levels of a straight-chain hydrocarbon nonacosane and in naringenin chalcone, an obligate compound in the flavonoid biosynthesis pathway.

病毒和扫描电容显微镜结合

Capacitances of five types of viruses, adenovirus type 5 (AV5), herpes simplex virus type 1 (HSV1), simian virus 40 (SV40), vaccinia (MVA), and cowpea mosaic virus (CPMV), were compared by AC capacitance scanning probe microscopy. This technique, using a Pt-coated AFM tip as an electrode to probe capacitance of materials between the tip and a bottom electrode, has been applied to study surface structures of semiconductors and polymers with nanometer spatial resolution; however, biological samples at the nanoscale have not been explored by this technique yet. Because most biological cells are poor conductors, this approach to probe electric properties of cells by capacitance is logical. This scanning probe technique showed that each virus has distinguishable and characteristic capacitance. A series of control experiments were carried out using mutant viruses to validate the origin of the characteristic capacitance responses for different viruses. A mutation on the capsid in HSV1 with green fluorescence proteins increased capacitance from 9  10-6 to 1  10-5 F/cm2 at the frequency of 104 Hz. Herpes simplex virus type 2 (HSV2) decreased capacitance when its envelope and glycoproteins were chemically extracted. These control experiments indicate that dielectric properties of capsid proteins and envelope glycoproteins significantly influence overall dielectric constants of viruses. Because those capsid proteins and glycoproteins are characteristic of the virus strain, this technique could be applied to detect and identify viruses at the single viron level using their distinct capacitance spectra as fingerprints without labeling.

利用原子力显微镜对活体细胞进行量子点注入

利用原子力显微镜对活体细胞进行量子点注入——在本文中作者利用生物型原子力显微镜，通过在碳纳米管表面修饰量子点的方法将量子点活体注入的细胞中。然后通过AR生物型原子力显微镜的强大荧光成像能力观察量子点注入效果。

内皮细胞原子力显微镜研究

内皮细胞原子力显微镜研究——本文利用原子力显微镜对液相培养液中的内皮细胞进行了结构研究。并利用其强大的力学成像功能研究了内皮细胞表面的弹性模量。

Nature杂志——结合光镊和原子力显微镜分析ZnO纳米线

结合光镊和原子力显微镜分析ZnO纳米线-通过光镊的高次谐波光学信号的探测，通过光镊操纵单根ZnO纳米线排列成一个阵列结构然后结合原子力显微镜的形貌分析能力。

针尖加强的近场光学显微镜

针尖加强的近场光学显微镜利用局域场增强原理克服传统的近场光学显微镜的光传输效率太低缺点。现在已经被广泛的应用于拉曼光谱、表面等离子体的探测中。而本文中提出了一个非常合理的针尖加强显微镜的模型，克服传统针尖加强显微镜结构复杂并且难以操作的困难。

全反射内置荧光显微镜结合原子力显微镜共同细胞成像

The atomic force microscope is a high-resolution scanning-probe instrument which has become an important tool for cellular and molecular biophysics in recent years but lacks the time resolution and functional specificities offered by fluorescence microscopic techniques. To exploit the advantages of both methods, here we developed a spatially and temporally synchronized total internal reflection fluorescence and atomic force microscope system. The instrument, which we hereby call STIRF-AFM, is a stage-scanning device in which the mechanical and optical axes are coaligned to achieve spatial synchrony. At each point of the scan the sample topography (atomic force microscope) and fluorescence (photon count or intensity) information are simultaneously recorded. The tool was tested and validated on various cellular (monolayer cells in which actin filaments and intermediate filaments were fluorescently labeled) and biomolecular (actin filaments and titin molecules) systems.Wedemonstrate that with the technique, correlated sample topography and fluorescence images can be recorded, soft biomolecular systems can be mechanically manipulated in a targeted fashion, and the fluorescence of mechanically stretched titin can be followed with high temporal resolution.

激光共聚焦显微镜与原子力显微镜相联用

多年以来，生物学家就一直将激光共聚焦显微镜应用于厚样本如细胞或组织的三维成像功能。原子力显微镜也采用了样品扫描过程来进行成像，且并不受光学衍射极限的限制。现在您可以把这些互补技术在MFP-3D-CF中完美的结合起来。这个新系统将MFP-3D-BIO的强大功能特征与您选择的商业化的激光共焦显微镜相结合。有了MFP-3D-CF，你就可以根据其荧光特性选择样本部位，然后用一个高分辨率的原子力显微镜扫描进行放大;将局部解剖与荧光性相结合;或用探针机械地刺激你的样品，测量光学反应。这一强有力的结合将给你的研究带来新的能力。

原子力显微镜和荧光显微镜共同使用

长期以来，生物学家一直对如何将原子力显微镜（ AFM ）和荧光显微镜（超短波）相结合很感兴趣。因为这两种显微镜都分别提供了独特的样品信息 荧光显微镜通常用来探测特定分子。不过这个显微技术经常有若干弊端，包括有限的空间分辨率和复杂的样品制备。同时这种显微镜的另一个局限是这种分子必须与荧光染料绑定，而这样会反过来会改变分子的状态。 另一方面，原子力显微镜是用悬臂扫描表面的一种测量方法。它提供的生物分子的三维分子分辨率无需额外的对比剂（如荧光色素，染料等），这样就无需迁就样本。而原子力显微镜也是一个持续时间长的较高分辨率的测量法。同时利用这些显微技术可为用户提供特定分子高分辨率的影像，也向各种研究试验开启了大门。在这一操作说明书中，我们将描述是用仪器和设置，样品制备，应用程序，例举这种技术的实验例子。

Nature杂志——内置全反射荧光显微镜结合原子力显微镜在单分子测试上的应用

在本文中着重介绍了宾夕法尼亚大学的纳米生物研究中（NIBC）结合AR公司的生物原子力显微镜和内置全反射荧光显微镜联用对实现单分子测试，这一全新发明为单分子研究打开了一个全新的方向。

Nature杂志——生物纳米学发展方向

光学显微镜在生物领域的应用已经趋于完美。生物领域的发展方向在哪？在更小的微观世界内，在本文中作者着重介绍了原子力显微镜在生物领域的应用和发展。同时指出了结合分子力测试的原子力系统将会在未来的生物学研究方面大方光彩。