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PreSens氧分析仪MCR-O1P1C1的工作原理介绍
氧分析仪MCR-O1P1C1的使用方法?
PreSensMCR-O1P1C1多少钱一台?
氧分析仪MCR-O1P1C1可以检测什么?
氧分析仪MCR-O1P1C1使用的注意事项?
PreSensMCR-O1P1C1的说明书有吗?
PreSens氧分析仪MCR-O1P1C1的操作规程有吗?
PreSens氧分析仪MCR-O1P1C1报价含票含运吗?
PreSensMCR-O1P1C1有现货吗?
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利用光学传感器在线测量摇瓶中的溶解氧
On-line measurement of dissolved O2 in shake-flasks was realized via immobilized sensor spots containing a fluorophore with an O2 dependent luminescent decay time. An unaffected sensor signal during 80 autoclaving cycles suggests multi-usage of sensor equipped shake-flasks. The sensor had a response time of 6 s. Quantification of gas-liquid mass transfer revealed maximum kLa values of 150 h−1, from which maximum O2 transfer capacity of 33 mM h−1 was calculated. Liquid volume and shaking frequency have a strong influence on kLa. Exemplified by cultivations of Corynebacterium glutamicum the importance of shaking rate for O2 supply of bacterial cultures is shown. Sampling of microbial cultures with intermittent shaking of a few minutes can cause O2 limitation. Based on the results of this work a simple and straightforward tool is now available for accurate O2 sensing in shake-flasks, which are widely used in microbial cultivations.
石油/化工
2017/11/16
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