2017/12/19 09:58
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产品配置单:
科捷紫外/UV1700系列/紫外可见分光光度计
型号: 紫外UV1700
产地: 江苏
品牌: 南京科捷
¥5000 - 9999
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山楂中含有多种黄酮类化合物。近年来的许多研究证明,山楂对心血管系统由降压、增加冠脉血流量、改善心肌供血供氧等功效,对高血压、高血脂、冠心病、心绞痛等症均有较好的疗效,是防治心血管疾病较好的天然植物资源,其重要有效成分——山楂总黄酮,据分析主要为牡荆碱鼠李糖苷、单乙酰牡荆碱鼠李糖苷、金丝桃苷及山楂酸等。山楂中黄酮类化合物是防治心血管疾病及降血脂的有效成分,由于各类黄酮物质的理化性质差别很大,因而检测手段也不可能统一。有关总黄酮的测定方法有:戴维斯法、薄层层析-比色法、微分脉冲极谱法、硝酸铝显色法、紫外分光光度法等。鲜山楂果胶含量达3.0%以上,但上述方法均忽略了山楂果胶对总黄酮含量的影响。本实验针对上述检测方法存在的问题,根据现有条件对硝酸铝显色-分光光度法进行改进,以芦丁为标准品,利用果胶酶将山楂果肉中的果胶分解后,再进行黄酮的含量分析,减少果胶对检测黄酮的影响,建立起更加精确的山楂总黄酮分析系统,旨在为山楂总黄酮的定量及生物功能评价提供检测方法。
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