葡萄糖(HK)检测试剂 glucose (HK) assay reagent 20 mL
葡萄糖标准品 glucose standard 0.5 mL
Description
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Application This kit is for the quantitative, enzymatic determination of glucose in food and other material. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phospho-gluconate in the presence of oxidized nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to glucose concentration.
Properties
usage sufficient for 20 assays
storage temp. 2-8°C
Safety
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26
References
reference Kunst, A., et al. Meth. Enzym. Anal. 3rd ed.,, 163-172, (1984)