TA仪器+差示扫描量热仪+NANO DSC

A pharmaceutical compound (10 mg/mL) was dissolved at pH 4 and at pH 10, then both samples were simultaneously heated in a MC-DSC. Thermal activity was evident, indicative of chemical reactions occurring in the samples. Under basic conditions the reaction was endothermic, while in acidic conditions the reaction was exothermic. When the logarithm of the thermal activity was plotted versus the reciprocal temperature, it became apparent that there were at least two reaction pathways with different activation energies at pH 10, while at pH 4 there was only evidence of a single degradative reaction. This information would be very useful in designing traditional HPLC stability screening studies since under basic conditions, this compound should clearly be stressed at lower rather than higher temperatures.

The development of monoclonal antibody (Mab) based drugs has undergone considerable growth since the release of the first Mab in the mid-eighties. Intuitive biophysical tools that examine and quantify interactions and conformational dynamics are important in demonstrating and understanding local and global conformational changes. The structure of antibodies as well as other macromolecules is related to its function and efficacy therefore control over these parameters is essential. The association of antigens to antibodies or of antibodies to delivery assemblies such as liposomes or gold nanoparticles can be addressed by isothermal titration calorimetry (ITC) while the folding of antibodies can be assayed using differential scanning calorimetry (DSC). Both techniques offer the advantage of using native solutions and both instruments are offered by TA Instruments.

Although membrane proteins are very difficult to work with, ultra-sensitive calorimetry can overcome the limitations of many other physical characterization techniques to provide insights into the factors driving membrane protein/lipid assembly and controlling the stability of the complexes.

A combination of HDX –MS and DSC is capable of revealing important structural details for biopharmaceuticals that cannot be obtained using other techniques HDX can map changes in binding regions and define structure of weak binding sites DSC characterization ensures highest quality structural data for differentiating weak binding molecular variants HDX-MS & DSC provide powerful, flexible analysis tools for DD, R&D and QC