2005/09/16 17:05
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产品配置单:
通微TriSep2100加压毛细管电色谱
型号: 2100
产地: 美国
品牌: 通微
¥20万 - 50万
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高效液相色谱仪EasySep®-3030
型号: EasySep®-3030
产地: 上海
品牌: 通微
¥10万 - 20万
参考报价
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方案详情:
NG-nitro-D-arginine (D-NNA), i.v. injected into rats, produced apressor response, and was presumed to act via chiral inversioninto NG-nitro-L-arginine (L-NNA), an inhibitor of nitric oxide synthase.We examined the possible role of renal D-amino acid
oxidase (DAAO) in the chiral inversion of D-NNA to L-NNA. Inpentobarbital-anesthetized rats, L-NNA was detected via capillary electrochromatography in the blood immediately after i.v.injection of D-NNA. The time course of appearance of L-NNAparalleled the increase in blood pressure elicited by D-NNA. Unilateral renal ligation partially, and bilateral ligation completely,blocked the pressor response as well as the conversionof D-NNA to L-NNA. Furthermore, injection into conscious rats
of sodium benzoate, a selective DAAO inhibitor, completely blocked the pressor response to naive D-NNA, but not pressor response to D-NNA preincubated with homogenates of the kidney. Homogenates of the kidneys, liver (lesser degree), and
brain (much lesser degree) converted D-NNA to L-NNA, and the chiral inversion was blocked by the addition of benzoate. Moreover,D-NNA chiral inversion correlates with the activity of DAAO. Our results reveal a novel pathway of chiral inversion of D-amino acids where the renal DAAO plays an essential role that accounts for the biological activity of D-NNA.
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