核心参数
应用级别: 实验室级别
仪器种类: 高压制备液相色谱
流速范围: 0.01-50 mL/min
流量精度: ≤0.3% RSD
流量重现性: ≤0.3% RSD
最大耐压: 420 bar
波长范围: 0.1 nm
波长重现性: -
基线噪声: 2.5 uAU
采集频率: 120Hz
安捷伦制备液相色谱1260 Infinity II Preparative的工作原理介绍
制备液相色谱1260 Infinity II Preparative的使用方法?
安捷伦1260 Infinity II Preparative多少钱一台?
制备液相色谱1260 Infinity II Preparative可以检测什么?
制备液相色谱1260 Infinity II Preparative使用的注意事项?
安捷伦1260 Infinity II Preparative的说明书有吗?
安捷伦制备液相色谱1260 Infinity II Preparative的操作规程有吗?
安捷伦制备液相色谱1260 Infinity II Preparative报价含票含运吗?
安捷伦1260 Infinity II Preparative有现货吗?
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基于分析方法优化制备方法的流程
In analytical HPLC good resolution is required over the entire gradient; in preparative HPLC it is only important to achieve good resolution around the target compound, which should be collected in highest purity. With a small set of optimized gradient methods a much better resolution can be achieved than with a single generic method. The generation of a small method set based on retention time windows in the pre-preparative analytical run is described in this Application Note. Using Agilent standards with known elution properties makes this an easy four-step process, which can be accomplished in less than one hour. An application example demonstrating how the resolution of the target compound can be improved is also shown.
制药/生物制药
2018/09/03
分析到制备的方法转换 – 聚焦梯度实现UHPLC到制备的方法放大
Synthesizing novel compounds or isolating natural products can be a laborious and time-consuming process. After analyzing the precious sample on an analytical UHPLC system, the crucial step is to transfer the method to a preparative system with a minimal risk of losing valuable work or collecting impure compounds. This Technical Overview describes a practical way to optimize the scale-up process for reversed-phase chromatography from an analytical UHPLC system to preparative LC systems using a focused gradient to increase the sample load on the preparative column to achieve optimum purity.
制药/生物制药
2018/09/03
ELSD触发制备—适用于4.6mm-75mm内径色谱柱的分流方案
This Technical Overview describes a solution to monitor and trigger the collection of preparative samples with the Agilent 1260 Infinity Evaporative Light Scattering Detector (ELSD) using a flow splitter. The Agilent 1260 Infinity ELSD is the detector of choice to monitor low UV-absorbing compounds or compounds with no UV-chromophore. Furthermore, this detection method is able to detect all compounds less volatile than the mobile phase, suitable for the detection of small molecule mixtures. A drug-like sample mixture has been purified on an Agilent 1260 Infinity Preparative-scale Purification System by triggering the fraction collection with the Agilent 1260 Infinity ELSD. 99 % of purity and 91 % recovery were achieved.
制药/生物制药
2018/09/03
使用自动纯化软件实现分析到质谱触发制备放大过程的自动化
The purification of a valuable product or product library after a long and complex synthesis is a very important process. The Agilent Automated Purifi cation Software introduces an Open-Access-based solution for isolating substances, with automated focused gradient to simplify the pre- and post-purification steps. This Technical Overview describes the easy steps to follow, from crude sample analytical scouting submission to pure target product archiving or screening. For example, a purification sample containing a target peak overlapped by an impurity was purifi ed on the Agilent 1260 Infinity Preparative-Scale LC/MS Purifi cation System using the Automated Purifi cation Software. This purifi cation produced a pure target compound with 88 % recovery, saving 40 % of solvent and runtime compared to a standard purifi cation method.
制药/生物制药
2018/09/03
ELSD触发制备的收集参数优化
On the Agilent OpenLAB CDS, fraction collection can be performed with a UV detector, or any additional detector, such as an Evaporative Light Scattering Detector (ELSD). In another Technical Overview1 , we described a simple way to split the preparative fl ow on the Agilent preparative LC systems to trigger the collection with an ELSD. This Technical Overview shows how to optimize peak-based fraction collection triggered by an ELSD. We describe the system setup, the delay calibration between detector and fraction collector, and the use of the variable light sensitivity of the Agilent 1260 Infi nity Evaporative Light Scattering Detector. We also discuss the peak-based fraction collection settings required to achieve the best purity and recovery.
制药/生物制药
2018/09/03
质谱触发制备的方法开发策略
Peak-based fraction collection triggered by a UV signal threshold leads to satisfactory results in most cases. Some samples and applications, however, require special attention when fraction collection parameters are set. This Technical Overview describes different strategies on how to achieve best collection results using different settings on an Agilent 1260 Infinity II Preparative LC/MSD System. UV and mass-based fraction collection, fraction pooling, as well as recovery collection are discussed in detail to provide knowledge that can be turned into successful and confident fraction collection.
制药/生物制药
2018/09/03
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制备型液相色谱纯化策略
在本篇白皮书中,安捷伦介绍了从分析级放大至制备型液相色谱的关键步骤概况。
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2022/08/11
选择指南:Agilent InfinityLab 液相色谱纯化解决方案
Agilent InfinityLab 液相色谱纯化解决方案是您从分析型到制备型工作流程的高性能仪器、色谱柱、软件,以及服务的不二之选。
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2022/08/11
以最高效率纯化样品
以最高效率纯化样品 Agilent InfinityLab 液相色谱纯化解决方案提供了适用于从分析型到制备型工作流程的仪器、色谱柱、软件和服务。
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2022/08/11
Agilent InfinityLab 液相色谱解决方案
可靠、高效、不断创新,为您提供最佳结果。获得更多关于 Agilent InfinityLab 液相色谱解决方案的全面的产品系列信息。
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2022/08/11
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