DETERMINATION OF ANIONS

IN FEEDSTUFFS, COMPOUND FEEDS, AND FEED RAW MATERIALS

Lumex Method M 04-73-2011

INTRODUCTION

The method  is used for the determination of the mass fraction of water-soluble chloride, nitrate, phosphate, and total sulfate in all types of feedstuffs, compound feeds, fodders, premixes, fodder additives, and feed raw materials (of plant, animal, and mineral origin) by capillary electrophoresis.

MEASUREMENT METHOD

The measurement method is based on capillary zone electrophoresis with indirect UV detection at the wavelength of 254 nm.

MEASUREMENT RANGE

The measurement ranges for the components are presented in the table below.

* For the sample weight of 100–500 mg.

The determination of phosphate is not hindered by the presence of carbonate (with mass concentration ratio of 10:1) and all other analytes (with mass concentration ratio of 1000:1). The determination is not hindered    by the presence of other inorganic anions, organic acids (acetic, citric, lactic, formic, oxalic) and neutral        organic substances.

EQUIPMENT AND REAGENTS

The CAPEL capillary electrophoresis system is used in measurements. Data  acquisition,  collection,  processing and output are performed using a personal computer running under WINDOWS® XP/7/8/10 operating system with installed dedicated software package ELFORUN.

Lumex Instruments set, order No 0300001921.

EXAMPLES OF REAL ANALYSES

BGE: chromate, with diethanolamine and CTA-OH

Capillary: L_eff/ L_tot  50/60 cm, ID 75 µm

Injection: 150 mbar x sec Voltage: – 25 kV Temperature: + 20 ^ОС Detection: 254 nm