Covalently coupling the antibody on an amine-self-assembled gold surface to probe hyaluronan-binding protein with capacitance measurement
2014-08-19 10:12
浏览:218次
分享:
资料摘要:
Hyaluronan-binding proteins (HABPs), the important structural components of extracellular matrices, served important structural and regulatory functions during development and in maintaining adult tissue homestats. A sensitive, specific and rapid-responsing immunosensor to probe hyaluronan-binding cartilage protein was presented in this work. The novel immunosensor supplied a label-free detection method for HABP, which was based on measuring the capacitance change in-between the unlabeled HABP (antigen) and rabbit-anti-HABP (Ra-HABP, antibody). The HABP immunosensor was prepared by covalently coupling Ra-HABP on an amine-self-assembled gold surface with glutaraldehyde. The capacitance change corresponding to the concentration of HABP, the target antigen, was evaluated by an electrochemical approach called potentiostatic-step in microseconds. The immunosensor showed a specific response to HABP in the range 10/1000 ng/ml. The presented work supplied a promising clinical screening method.
下载本篇资料:
ovalentlycouplingtheantibodyonanamineselfassembled.pdf
214KB
相关资料
瑞士万通OMNIS奥秘一代全新电位滴定平台
包含OMNIS详细的主要功能和特点
以及各种仪器参数等等
简单 经济 耐用
?智能型离子色谱系统
?操作便捷,性能稳定
?测定结果准确可靠
?测定各种样品中的阴离子、阳离子及其它极性物质
腐蚀的基本概念--腐蚀主要就是金属的退化和钝化,最主要的一个例子就是钢中贴的腐蚀,大部分腐蚀属于电化学反应......
加100mL无隔膜库仑法醛酮试剂于滴定杯中,并滴定至无水(预滴定)。用注射器加入样品适量,测定水分。测定酮类样品的水分,要使用库仑法醛酮专用试剂