Optimizing Chiral Separations and Purification of

Pharmaceutical laboratories, as part of a chiral drug investigation, may be asked to
provide separations of all chiral compounds to facilitate the testing of individual
enantiomers (1). Sample cleanup prior to purification is a factor in reducing the cost
and time of semi‐preparative purification, resulting from the typical high cost and
fragility of chiral sorbents. To eliminate exhaustive sample cleanup procedures, a
complete automation of the analyte purification and chiral separation was
developed. The application principle injected samples onto a separation column to
cleave individual peaks and transfer these peaks onto secondary dual chiral columns
for separation. Automated and complete purification of analytes with high
resolution of the separated enantiomers provided speedy purification, efficient
separation, and simplified collection of chiral analytes, eliminating the need for
extensive sample cleanup.