应用SPRimager II及SpotReady芯片监测DNA-DNA相互作用
In this experment, two thiol-modified DNA oligomers are covalently immobilized on the gold surface of SpotReady chips. Specific binding of DNA complementay to one of the oligomers is monitored on the SPRimager II. In addition to DNA oligomers, this probe immobilization protocol can be used to attach any thiol modified probe to gold, such as thiolated RNA oligomers or peptides synthesized with a terminal cysteine. As an alternative, oligonucleotide arrays can instead be prepared on s streptavidin surface using biotinylated oligomers as probes.