简介:This application note shows that the epMotion family of
automated liquid handling systems is ideal for reliable and reproducible setup of sensitive real-time quantitative PCR (qPCR) assays in daily routine, without the 简介:This application note shows that the epMotion family of
automated liquid handling systems is ideal for reliable and reproducible setup of sensitive real-time quantitative PCR (qPCR) assays in daily routine, without the risk of cross contamination. The complete workflow from Master Mix
preparation to 96-well PCR plate setup was done with the
automation system. Cross contamination assay in chess
board pattern was performed according to FDA guidance.
Runs on two independent epMotion systems with 2 plates
each on 3 days are compared in this document.
The assay reproducibility was excellent with low coefficients of variation for both intra and inter-plate comparisons of Ct values. Automated assay setups were carried with the epMotion 5073 and the 5075 systems.详细>