简介:2.1 A small volume of aqueous sample, typically 2 to 3 mL, is injected into an ion
chromatograph to flush and fill a constant-volume sample loop. The sample is then injected into
a flowing stream of carbonate-bicarbona简介:2.1 A small volume of aqueous sample, typically 2 to 3 mL, is injected into an ion
chromatograph to flush and fill a constant-volume sample loop. The sample is then injected into
a flowing stream of carbonate-bicarbonate eluent.
2.2 The sample is pumped through two different ion exchange columns, then a suppressor
device, and into a conductivity detector. The two ion exchange columns, a precolumn or guard
column and a separator column, are packed with low-capacity, strongly basic anion exchange resin.
Ions are separated into discrete bands based on their affinity for the exchange sites of the resin.
The suppressor is an ion exchange-based device that reduces the background conductivity of the
eluent to a low or negligible level and also converts the anions in the sample to their more
conductive acid forms. The separated anions in their acid forms are measured using an electricalconductivity
cell. Anions are identified based on their retention times compared to known
standards. Quantitation is accomplished by measuring the peak height or area and comparing it
to a calibration curve generated from known standards.详细>