简介:2.1 Test kits are commercially available for this method. The manufacturer's directions
should be followed.
2.2 In general, the method is performed using an extract of a solid sample. Solid samples
are prepared by ext简介:2.1 Test kits are commercially available for this method. The manufacturer's directions
should be followed.
2.2 In general, the method is performed using an extract of a solid sample. Solid samples
are prepared by extraction with a mixture of hydrochloric and nitric acids for ten minutes and then
buffered prior to analysis. The sample is added to a tube (treated with BSA-glutathione) and
incubated at ambient temperatures for five minutes. The mercuric ions bound to the sulflhydryl
groups of the BSA-glutathione are now reacted with a reconstituted antibody specific for mercury
and incubated for five more minutes. A peroxidase conjugate is added to the sample, reacting with
any mercury specific antibody. The substrate is then added forming a color that is in proportion to
the amount of mercury originally present in the sample. The color produced is then
spectrophotometrically compared with the control standards.详细>