简介:2.1 The reporter gene system utilizes a human cell line (101L) into which a plasmid
containing a human CYP1A1 promoter and 5'-flanking sequences fused to a reporter gene,
firefly luciferase, have been stably integrated简介:2.1 The reporter gene system utilizes a human cell line (101L) into which a plasmid
containing a human CYP1A1 promoter and 5'-flanking sequences fused to a reporter gene,
firefly luciferase, have been stably integrated. In the presence of CYP1A1-inducing compounds,
the enzyme luciferase is produced, and its reaction with luciferin can be detected by measuring
relative light units (RLUs) in a luminometer. CYP1A1-inducing environmental contaminants
include polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs),
coplanar polychlorinated biphenyl congeners (PCBs), and high molecular weight polynuclear
aromatic hydrocarbons (PAHs).详细>