简介:Metabolic profiling and structural elucidation of possible
metabolites via LC/MS/MS was simplified and accelerated
by Fragment Ion Search (FISh) processing in Mass Frontier
7.0 software. This workflow can be combin简介:Metabolic profiling and structural elucidation of possible
metabolites via LC/MS/MS was simplified and accelerated
by Fragment Ion Search (FISh) processing in Mass Frontier
7.0 software. This workflow can be combined with
Multiple Mass Defect Filtering (MMDF) in MetWorks 1.3
software for reduced processing times without incurring
false negatives. The availability of high-resolution,
accurate mass LC/MS/MS data minimizes the possibility of
false positives in the generated list of related components.
All known metabolites of ticlopidine in a S9 human liver
fraction incubation were detected and identified using this
automated processing workflow, including the unusual
S-oxide dimer metabolite. This is interesting to note
because FISh was able to provide an indication for the
presence of this metabolite just through the identification
of an in-source fragment.详细>