简介:2.1 This method describes procedures for Florisil cleanup of solvent extracts of
environmental samples. It provides the option of using either traditional column chromatography
techniques to solid-phase extraction cart简介:2.1 This method describes procedures for Florisil cleanup of solvent extracts of
environmental samples. It provides the option of using either traditional column chromatography
techniques to solid-phase extraction cartridges. Generally, the traditional column chromatography
technique uses larger amounts of adsorbent and, therefore, has a greater cleanup capacity.
2.2 In the column cleanup protocol, the column is packed with the required amount of
adsorbent, topped with a water adsorbent, and then loaded with the sample extract. Elution of the
analytes is effected with a suitable solvent(s), leaving the interfering compounds on the column.
The eluate may be further concentrated prior to gas chromatographic analysis.
2.3 The cartridge cleanup protocol uses solid-phase extraction cartridges containing 40
µm particles of Florisil (60 D pores). Each cartridge is washed with solvent immediately prior to use.
The sample extract is loaded onto the cartridge which is then eluted with suitable solvent(s). A
vacuum manifold is required to obtain reproducible results. The eluate may be further concentrated
prior to gas chromatographic analysis.
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