简介:Fluorescence microscopy is the most fundamental measurement procedure of a laser scanning microscope. In fluorescence microscopy, the intensity of the detected fluorescence emission is used to monitor chemical reactions 简介:Fluorescence microscopy is the most fundamental measurement procedure of a laser scanning microscope. In fluorescence microscopy, the intensity of the detected fluorescence emission is used to monitor chemical reactions or to localise the fluorophore in imaging experiments.The fluorescence intensity is, however, not the only parameter that can be monitored. Even more information is available by looking at the temporal characteristics of the fluorescence emission with time-resolved techniques such as fluorescence lifetime imaging (FLIM) or fluorescence correlation spectroscopy (FCS).详细>