简介:Human Hepcidin
FOR RESEARCH USE ONLY
Assay range:10μg/L -150μg/L 96 determinations
Purpose
This kit allows for the determination of Hepcidin concentrations in Human serum, cell culture supernates a简介:Human Hepcidin
FOR RESEARCH USE ONLY
Assay range:10μg/L -150μg/L 96 determinations
Purpose
This kit allows for the determination of Hepcidin concentrations in Human serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Human Hepcidin level in the sample, use Purified Human Hepcidin antibody to coat microtiter plate wells, make solid-phase antibody, then add Hepcidin to wells, Combined Hepcidin antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Hepcidin in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>