简介:Human ZNT8 Ab
FOR RESEARCH USE ONLY
Assay range:6.0ng/L – 160 ng/L 96 determinations
Purpose
This kit allows for the determination of ZNT8 Ab concentrations in Human serum, cell culture supernates 简介:Human ZNT8 Ab
FOR RESEARCH USE ONLY
Assay range:6.0ng/L – 160 ng/L 96 determinations
Purpose
This kit allows for the determination of ZNT8 Ab concentrations in Human serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Human ZNT8 Ab IgM level in the sample, use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add ZNT8 Ab to wells, Combined antigen which With HRP labeled, become antigen – antibody - enzyme-antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human ZNT8 Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>