简介:Human Ep-CAM/CD362
FOR RESEARCH USE ONLY
Assay range:1.2ng/L -45ng/L 96 determinations
Purpose
This kit allows for the determination of Ep-CAM/CD362 concentrations in Human serum, cell culture supe简介:Human Ep-CAM/CD362
FOR RESEARCH USE ONLY
Assay range:1.2ng/L -45ng/L 96 determinations
Purpose
This kit allows for the determination of Ep-CAM/CD362 concentrations in Human serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Human Ep-CAM/CD362 level in the sample, use Purified Human Ep-CAM/CD362 antibody to coat microtiter plate wells, make solid-phase antibody, then add Ep-CAM/CD362 to wells, Combined Ep-CAM/CD362 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Ep-CAM/CD362 in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>