简介:The kit assay Human GAD-Ab level in the sample, use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add GAD-Ab to wells, Combined antigen which With HRP labeled, become antigen – antibody 简介:The kit assay Human GAD-Ab level in the sample, use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add GAD-Ab to wells, Combined antigen which With HRP labeled, become antigen – antibody - enzyme-antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human GAD-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>