简介:Principle of the assay
The kit assay SBA level in the sample,use Purified SBA antibody to coat microtiter plate wells, make solid-phase antibody, then add SBA to wells, Combined SBA antibody which With HRP labeled,becom简介:Principle of the assay
The kit assay SBA level in the sample,use Purified SBA antibody to coat microtiter plate wells, make solid-phase antibody, then add SBA to wells, Combined SBA antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SBA in the samples is then determined by comparing the O.D. of the samples to the standard curve.详细>