简介:2-D Gel Protocol for Pseudomonas aeruginosa
(modified from Elinor Pulcini’s protocol)
1. Protein Assay (Modified Lowry Protein Assay or BCA Protein Assay)
2. Solubilizing and Precipitating Proteins
3. Rehydrating简介:2-D Gel Protocol for Pseudomonas aeruginosa
(modified from Elinor Pulcini’s protocol)
1. Protein Assay (Modified Lowry Protein Assay or BCA Protein Assay)
2. Solubilizing and Precipitating Proteins
3. Rehydrating Isoelectricfocusing strips for First Dimension
4. Isoelectric Focusing (IEF) First Dimension
5. Pouring Gels for SDS-PAGE Second Dimension
6. Loading and running Gels for SDS-PAGE- Second Dimension
7. Unloading of Gels from DALT Tank
8. Staining of the Gels
Solutions and Stocks for Proteomic Procedures
IEF and IPG Strip Preparation Procedures
2-D Gel Procedures
Staining Procedure
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