简介:Goat interleukin 1(IL-1)
FOR RESEARCH USE ONLY
Assay range:1.2ng/L -50 ng/L 96 determinations
Purpose
This kit allows for the determination of IL-1 concentrations in Goat serum, cell
culture supernates and other bio简介:Goat interleukin 1(IL-1)
FOR RESEARCH USE ONLY
Assay range:1.2ng/L -50 ng/L 96 determinations
Purpose
This kit allows for the determination of IL-1 concentrations in Goat serum, cell
culture supernates and other biological fluids
Principle of the assay
The kit assay Goat IL-1 level in the sample,use Purified Goat IL-1 antibody to coat
microtiter plate wells, make solid-phase antibody, then add IL-1 to wells, Combined IL-1
antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after
washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of Goat IL-1 in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
Materials provided with the kit
1 wash solution 20ml×1bottle详细>