简介: Rat Estradiol(E2) ELISA Kit
FOR RESEARCH USE ONLY
Assay range:3ng/L -80ng/L 96 determinations
Purpose
This kit allows for the determination of E2 concentrations in Rat serum, cell culture supernates 简介: Rat Estradiol(E2) ELISA Kit
FOR RESEARCH USE ONLY
Assay range:3ng/L -80ng/L 96 determinations
Purpose
This kit allows for the determination of E2 concentrations in Rat serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Rat E2 level in the sample,use Purified Rat E2 antibody to coat microtiter plate wells, make solid-phase antibody, then add E2 to wells, Combined E2 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat E2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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