简介:Rat IL-10
FOR RESEARCH USE ONLY
Assay range:3 ng/L -100 ng/L 96 determinations
Purpose
This kit allows for the determination of IL-10 concentrations in Rat serum, cell
culture supernates and other biological fluids
简介:Rat IL-10
FOR RESEARCH USE ONLY
Assay range:3 ng/L -100 ng/L 96 determinations
Purpose
This kit allows for the determination of IL-10 concentrations in Rat serum, cell
culture supernates and other biological fluids
Principle of the assay
The kit assay Rat IL-10 level in the sample, use Purified Rat IL-10 antibody to coat
microtiter plate wells, make solid-phase antibody, then add IL-10 to wells, Combined IL-10
antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after
washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of Rat IL-10 in the详细>