简介: Rat MURF1
FOR RESEARCH USE ONLY
Assay range: 5 ng/L - 180 ng/L 96 determinations
Purpose
This kit allows for the determination of MURF1 concentrations in Rat serum, cell culture supernates and ot简介: Rat MURF1
FOR RESEARCH USE ONLY
Assay range: 5 ng/L - 180 ng/L 96 determinations
Purpose
This kit allows for the determination of MURF1 concentrations in Rat serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Rat MURF1 level in the sample, use Purified Rat MURF1 antibody to coat microtiter plate wells, make solid-phase antibody, then add MURF1 to wells, Combined MURF1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat MURF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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