简介:Rat tumor necrosis factor α(TNF-α)ELISA Kit
FOR RESEARCH USE ONLY
INTRODUCTION
Assay range:10ng/L-360ng/L 96 determinations
Purpose
This kit allows for the determination of TNF- α concentrations in Rat
serum, cell 简介:Rat tumor necrosis factor α(TNF-α)ELISA Kit
FOR RESEARCH USE ONLY
INTRODUCTION
Assay range:10ng/L-360ng/L 96 determinations
Purpose
This kit allows for the determination of TNF- α concentrations in Rat
serum, cell culture supernates and other biological fluids
PRINCIPLE OF TEST
The kit assay Rat TNF- α level in the sample , use Purified Rat TNF- α
antibody to coat microtiter plate wells, make solid-phase antibody, then add
TNF- α to wells, Combined TNF- α antibody which With HRP labeled, become
antibody - antigen - enzyme-antibody complex, after washing Completely, Add
TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of TNF- α in the samples is then
determined by comparing the O.D. of the samples to the standard curve.详细>