简介:Rat Interleukin6 (IL-6)ELISA Kit
FOR RESEARCH USE ONLY
INTRODUCTION
Assay range:3pg/ml-120pg/ml 96 determinations
Purpose
This kit allows for the determination of IL-6 concentrations in Rat
serum, cell culture supe简介:Rat Interleukin6 (IL-6)ELISA Kit
FOR RESEARCH USE ONLY
INTRODUCTION
Assay range:3pg/ml-120pg/ml 96 determinations
Purpose
This kit allows for the determination of IL-6 concentrations in Rat
serum, cell culture supernates and other biological fluids
PRINCIPLE OF TEST
The kit assay Rat IL-6 level in the sample, use Purified Rat IL-6 antibody
to coat microtiter plate wells, make solid-phase antibody, then add IL-6 to wells,
Combined IL-6 antibody which With HRP labeled, become antibody - antigen -
enzyme-antibody complex, after washing Completely, Add TMB substrate
solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,
reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of Rat IL-6 in the samples is then determined by comparing the
O.D. of the samples to the standard curve.详细>