简介:Rat Angiopoietin-1 (Ang-1)
FOR RESEARCH USE ONLY
Assay range:50pg/ml- 2600pg/ml 96 determinations
Purpose
This kit allows for the determination of Ang-1 concentrations in Rat serum, cell
culture supernates and other简介:Rat Angiopoietin-1 (Ang-1)
FOR RESEARCH USE ONLY
Assay range:50pg/ml- 2600pg/ml 96 determinations
Purpose
This kit allows for the determination of Ang-1 concentrations in Rat serum, cell
culture supernates and other biological fluids
Principle of the assay
The kit assay Rat Ang-1 level in the sample, use Purified Rat Ang-1 antibody to coat
microtiter plate wells, make solid-phase antibody, then add Ang-1 to wells, Combined Ang-1
antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after
washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of Rat Ang-1 in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
Materials provided with the ki详细>