简介:Rat Activated protein C(APC)ELISA Kit
FOR RESEARCH USE ONLY
INTRODUCTION
Assay range:0.1 ng/ml - 4 ng/ml 96 determinations
Purpose
This kit allows for the determination of APC concentrations in Rat
serum, cell cult简介:Rat Activated protein C(APC)ELISA Kit
FOR RESEARCH USE ONLY
INTRODUCTION
Assay range:0.1 ng/ml - 4 ng/ml 96 determinations
Purpose
This kit allows for the determination of APC concentrations in Rat
serum, cell culture supernates and other biological fluids
PRINCIPLE OF TEST
The kit assay Rat APC level in the sample, use Purified Rat APC
antibody to coat microtiter plate wells, make solid-phase antibody, then add
APC to wells, Combined APC antibody which With HRP labeled, become
antibody - antigen - enzyme-antibody complex, after washing Completely, Add
TMB substrate solution,TMB substrate becomes blue color At HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of Rat APC in the samples is then详细>