简介: Goat Anti-Guinea Pig Tumor Necrosis Factor α
Collect Sample –Serum or blood plasma
Storage: 2 - 8 °C Packa简介: Goat Anti-Guinea Pig Tumor Necrosis Factor α
Collect Sample –Serum or blood plasma
Storage: 2 - 8 °C Package size: 96 determinations
PRINCIPLE OF THE METHOD
The TNF-a kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known TNF-a concentrations and unknowns are pipetted into these wells. During the first incubation, the TNF-a antigen and a biotinylated monoclonal antibody specific for TNF-a are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured 详细>