简介:Goat anti- Rabbit Endothelin-1
Collect Sample – serum or blood plasma
Storage: 2 - 8 °C Package size: 96 de简介:Goat anti- Rabbit Endothelin-1
Collect Sample – serum or blood plasma
Storage: 2 - 8 °C Package size: 96 determinations
PRINCIPLE OF THE METHOD
The ET-1 kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known ET-1 concentrations and unknowns are pipetted into these wells. During the first incubation, the ET-1 antigen and a biotinylated monoclonal antibody specific for ET-1 are simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ET-1 present in the samples.
详细>