简介:PerkinElmer的用户手册——荧光光谱导论
An Introduction to Fluorescence Spectroscopy
Table of Contents
Table of Contents............................................................................................3 简介:PerkinElmer的用户手册——荧光光谱导论
An Introduction to Fluorescence Spectroscopy
Table of Contents
Table of Contents............................................................................................3
Luminescence and the nature of light.............................................................5
Fluorescence...................................................................................................7
Phosphorescence...........................................................................................11
Light scattering.............................................................................................14
Rayleigh-Tyndall scattering..................................................................14
Raman scattering...................................................................................14
Quantitation..................................................................................................16
Sensitivity.....................................................................................................18
Instrumental sensitivity.........................................................................18
Method sensitivity.................................................................................18
Absolute sensitivity...............................................................................18
Instrumentation.............................................................................................21
General..................................................................................................21
Light sources.........................................................................................22
Wavelength selection.............................................................................24
Detectors................................................................................................25
Read-out devices...................................................................................26
Sample holders......................................................................................26
Problems of high blank values......................................................................28
Cuvettes.................................................................................................28
Solvents and Reagents...........................................................................28
Other contaminants................................................................................29
Working with dilute solutions......................................................................30
General..................................................................................................30
Adsorption.............................................................................................30
Photo-decomposition.............................................................................31
Oxidation...............................................................................................31
Factors affecting quantitative accuracy........................................................32
Non-linearity..........................................................................................32
Temperature effects...............................................................................32
pH effects...............................................................................................33
Inner-filter effects..................................................................................33
Quenching..............................................................................................33
References....................................................................................................34
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