简介:利用RP-HPLC分析、纯化蛋白质和多肽的手册Table of Contents
Introduction . .2
Mechanism of Interaction . .4
The Role of the Column in Polypeptide Separations . .8
Analytical Conditions: The Role of the Mobile Phase and 简介:利用RP-HPLC分析、纯化蛋白质和多肽的手册Table of Contents
Introduction . .2
Mechanism of Interaction . .4
The Role of the Column in Polypeptide Separations . .8
Analytical Conditions: The Role of the Mobile Phase and Temperature .17
Reversed-Phase HPLC/Mass Spectrometry . .26
The Role of Reversed-Phase HPLC in Proteomic Analysis . .30
Examples of the Use of Reversed-Phase HPLC
in the Analysis of Polypeptides .32
HPLC as a Tool to Purify and Isolate Polypeptides . .40
Viral Inactivation During Reversed-Phase HPLC Purification . .50
Appendices
Appendix A: Column Characteristics . .52
Appendix B: The Care and Maintenance of Reversed-Phase Columns . .53
Appendix C: The Effect of Surfactants on Reversed-Phase Separations . .56
Appendix D: Ion Exchange Chromatography:
Orthogonal Analytical Techniques .. .58
Appendix E: The Effect of System Hardware
on Reversed-Phase HPLC Polypeptide Separations . .60
Technical References . .62详细>