简介:Vitamin E or a-tocopherol is a well-known antioxidant that is added to many foods to reduce oxidation. It is
also marketed in vitamin formulas and as a nutraceutical. Many natural products such as soybeans, corn,
saffl简介:Vitamin E or a-tocopherol is a well-known antioxidant that is added to many foods to reduce oxidation. It is
also marketed in vitamin formulas and as a nutraceutical. Many natural products such as soybeans, corn,
safflowers, peanuts, wheat germ and sunflowers contain tocopherols in low concentration. Because of the
high market value placed on natural tocopherols, their isolation from natural sources in a highly purified form
is desired. The oily nature of these natural product matrices creates problems when isolating tocopherols.
Direct injections of neat oil or oil dilutions of natural products onto reversed phase HPLC columns cause
many problems including coating the stationary phase with lipids that leads to ghost peaks, unstable baselines
and short lifetimes. Using reversed phase flash chromatography as the preliminary isolation step allows the
tocopherols to be concentrated and have fewer oils as contaminants thereby increasing the lifetime of the
HPLC columns.详细>