简介:This application note describes the use of a quick, easy, cheap, effective, rugged, and
safe (QuEChERS) AOAC sample preparation approach for extraction and cleanup of
17 GC-amenable pesticide residues from multiple cla简介:This application note describes the use of a quick, easy, cheap, effective, rugged, and
safe (QuEChERS) AOAC sample preparation approach for extraction and cleanup of
17 GC-amenable pesticide residues from multiple classes, in apple. The method
employed involves initial extraction in a buffered aqueous/acetonitrile system, an
extraction/ partitioning step after the addition of salt, and a cleanup step utilizing dispersive
solid phase extraction (dispersive SPE). The two different dispersive SPE
clean-up approaches used either a 1 mL or 8 mL sample volume and were evaluated
in parallel after sample extraction. The target pesticides in the apple extracts were
then analyzed by gas chromatography/mass spectrometry (GC/MS) operating in
selective ion monitoring (SIM) mode. The method was validated in terms of recovery
and reproducibility. The limit of quantitation (LOQ) for most pesticides is 10 ng/g;
however, the pesticide Folpet has an LOQ of 50 ng/g in apple. This application
employing SampliQ QuEChERS kits produced results well below the maximum residue
limits (MRLs) for all the pesticides screened. The spiked levels for the recovery experiments
were 10, 50, and 200 ng/g. Recoveries ranged between 70 and 136% (92.5% on
average), with RSD below 15% (5.0% on average).
Authors
Limian Zhao, David Schultz, and
Joan Stevens
Agilent Technologies, Inc.
2850 Centerville Road
Wilmington, DE 19809-1610
USA
Application Note
Food Safety详细>