Detection of residual bacitracin A, colistin A, and colistin B in milk by TSQ Discovery.pdf
简介:A method based on liquid chromatography tandem mass
spectrometry for simultaneous determination of residual
bacitracin A, colistin A, and colistin B in milk and animal
tissues was described. Validation data including 简介:A method based on liquid chromatography tandem mass
spectrometry for simultaneous determination of residual
bacitracin A, colistin A, and colistin B in milk and animal
tissues was described. Validation data including precision,
in terms of intra-day and inter-day recoveries, LOQ, LOD,
and quality control measures were discussed in detail.
Sample cleanup using a more efficient SPE cartridge
resulted in comprehensive removal of the interfering
substances that had been found to cause ion signal
suppression during mass detection in a previous study.
The method offers broad application in detecting the
residual polypeptides in poultry and porcine; and allows
the employment of calibration standards, instead of matrix
spikes, for quantification purpose with improved detection
limits, accuracy, and precision. Furthermore, by using a
one-point control approach, the method provides a rapid
and high-throughput method for surveillance exercises.
Notwithstanding the above, the previous method [17]
could still be used to quantify bacitracin A and colistin A in bovine milk and offal samples; and also it might be used, if
appropriate, as a cross-checking technique with the present
method for the presence of the concerned analytes in
bovine samples.详细>