简介:We used laser nanodissection to study the nature, magnitude and distribution of forces at cell-cell contacts during tissue
morphogenesis in live Drosophila embryos. We designed two set-ups coupling a near infrared femto简介:We used laser nanodissection to study the nature, magnitude and distribution of forces at cell-cell contacts during tissue
morphogenesis in live Drosophila embryos. We designed two set-ups coupling a near infrared femtosecond laser to
either an inverted fluorescence microscope or a spinning-disk confocal microscope. We show that the developed tools
are able to locally disrupt the acto-myosin network while preserving the integrity of the membranes. With these systems,
we could explore the redistribution of cortical elements and relaxation of cell-cell contacts after local ablation.详细>