简介:Second-harmonic generation (SHG) and two-photon excitation
fluorescence (TPEF) are relatively new and promising tools for
the detailed imaging of biological samples and processes at the microscopic
level. By exploitin简介:Second-harmonic generation (SHG) and two-photon excitation
fluorescence (TPEF) are relatively new and promising tools for
the detailed imaging of biological samples and processes at the microscopic
level. By exploiting these nonlinear phenomena phototoxicity
and photobleaching effects on the specimens are reduced dramatically.
The main target of this work was the development of a
compact inexpensive and reliable experimental apparatus for nonlinear
microscopy measurements. Femtosecond laser pulses were utilized
for excitation. We achieved high-resolution imaging and mapping
of Caenorhabditis elegans (C. elegans) neurons and muscular
structures of the pharynx, at the microscopic level by performing SHG
and TPEF measurements. By detecting nonlinear phenomena such as
SHG and TPEF it is feasible to extract valuable information concerning
the structure and the function of nematode neurons.详细>