简介:Mass spectrometry has become an important technique to correlate proteins to their genes. This has been achieved,
in part, by improvements in ionization and mass analysis techniques concurrently with large-scale DNA seq简介:Mass spectrometry has become an important technique to correlate proteins to their genes. This has been achieved,
in part, by improvements in ionization and mass analysis techniques concurrently with large-scale DNA sequencing
of whole genomes. Genome sequence information has provided a convenient and powerful resource for protein
identiÐcation using data produced by matrix-assisted laser desorption/ionization time-of-Ñight (MALDI/TOF) and
tandem mass spectrometers. Both of these approaches have been applied to the identiÐcation of electrophoretically
separated protein mixtures. New methods for the direct identiÐcation of proteins in mixtures using a combination
of enzymatic proteolysis, liquid chromatographic separation, tandem mass spectrometry and computer algorithms
which match peptide tandem mass spectra to sequences in the database are also emerging. This tutorial review
describes the principles of ionization and mass analysis for peptide and protein analysis and then focuses on current
methods employing MALDI and electrospray ionization for protein identiÐcation and sequencing. Database
searching approaches to identify proteins using data produced by MALDI/TOF and tandem mass spectrometry
are also discussed详细>