Impact of column peak capacity on peptide mapping by reversed-phase high-performance liquid chromatography (RP-HPLC)
简介:Peptide mapping by RP-HPLC is often used for quality control therapeutic proteins. Since tryptic digestion generates a complex mixture of peptides (typically 25-100, depending on the size of the protein), it is difficult简介:Peptide mapping by RP-HPLC is often used for quality control therapeutic proteins. Since tryptic digestion generates a complex mixture of peptides (typically 25-100, depending on the size of the protein), it is difficult to achieve an ideal separation of all peptides of interest.详细>