简介:关键词:HPLC-ELSD;黄芪提取物;黄芪甲苷;喷雾干燥
摘要:
目的: 研究喷雾干燥法制备黄芪提取物中黄芪甲苷的HPLC-ELSD测定方法。
方法:黄芪醇提液经浓缩后喷雾干燥,采用蒸发光散射检测器(ELSD)以黄芪甲苷为对照品对黄芪提取物中的黄简介:关键词:HPLC-ELSD;黄芪提取物;黄芪甲苷;喷雾干燥
摘要:
目的: 研究喷雾干燥法制备黄芪提取物中黄芪甲苷的HPLC-ELSD测定方法。
方法:黄芪醇提液经浓缩后喷雾干燥,采用蒸发光散射检测器(ELSD)以黄芪甲苷为对照品对黄芪提取物中的黄芪甲苷进行HPLC分析,色谱柱:Elite-ODS,流动相∶乙腈-水(36∶64),流速:1.0ml/min,ELSD参数:漂移管温度为105°C,N2流速为2.84ml/min。
结果:按选定的色谱条件测定,线性良好,平均加样回收率达91.68%,RSD 为1.64%,实验还发现喷雾干燥过程中加入可溶性淀粉有助于粉末形成,但对含量测定有影响。
结论:本方法对黄芪甲苷的测定良好,应寻找更好的喷雾干燥助剂。
中图分类号:R284.2 文献标识码:A 文章编号:1001-1528(2001)-0673-02
Determination of Astragloside IV in Extraction of Radix Astragali by HPLC-ELSD
WU Yong-ping,XU Yong-mei,CAO Yuan,CAO Zheng-zhong
(Jiangsu Provincial Institute of Materia Medica, Jiangsu Nanjing 210009, China)
Key words: HPLC-ELSD; extractive of Radix Astragali; Astragaloside IV; spray-dry
Abstract:
Objective: To develop a method to determine Astragaloside IV in spray-dry of Rad ix Astragali by HPLC-ELSD.
Methods: ELSD was used to determine directly Astragaloside IV in sp ray-dry of Radix Astragali on Elite-OSD column, using CH3CN-H 2O(36∶64) as a mobile phase. The flow rate was 1.0 ml/min. The temperature of drift tube for ELSD was 105°C and the flow rate of N2 was 2.84 ml/min.
Results: The recovery of the added sample was 91.68% and RSD 1.6 4 %. Soluble amylum was helpful for spray-dry but it can influence was determinat ion of Astragaloside IV.
Conclusion: The method is accurate and can be used in the determination of Astragaloside IV in spray-dry of Radix Astragali. Better adjuvant should be found.
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