简介:GFP (Green Fluorescent Protein) is an extremely stable protein of 2
38 amino acids produced by the jellyfish Aequorea victoria1 that fl
uoresces in the lower green region of the visible spectrum. Over th
e past few ye简介:GFP (Green Fluorescent Protein) is an extremely stable protein of 2
38 amino acids produced by the jellyfish Aequorea victoria1 that fl
uoresces in the lower green region of the visible spectrum. Over th
e past few years GFP has become one of the most widely used tools i
n molecular and cell biology as the GFP gene can be expressed in no
n-homologous species. Additionally, the fact that GFP can generate
a highly visible fluorescence that requires no additional substrate
s has made it valuable as a marker for gene expression, as a cell l
ineage tracer or as an in vivo covalently attached fluorescent labe
l replacing more invasive and unwieldy fluorescent staining techniq
ues. GFP can be monitored non-invasively and may be used as a quant
itative marker in living cells. GFP can also be used as a tag for
studying real time localization and movement of protein-GFP chimera
s within cells2.详细>