PepSwift 生物色谱柱 164585

PepSwift 生物色谱柱

对于自下而上的蛋白质识别速度快，高分辨率的LC / MS分离，生物标志物发现和系统生物学应用程序，使用的Thermo Scientific PepSwift和毛细管液相色谱柱。基于聚苯乙烯二乙烯基苯共聚物，整体结构提供了高品质的替代传统微粒吸附剂，以色谱分离，提供重要的优点。高灵敏度蛋白质组学和生物技术的应用很容易使用这些列执行。

PepSwift 生物色谱柱

Monolithic Columns for Fast, High-Resolution Protein and Peptide Analysis

PepSwift monolithic columns are specially designed for fast separation and identification of proteins and peptides using nano and capillary LC coupled to MS. PepSwift columns are based on a polystyrene divinylbenzene copolymer bed. The bed structure of the column offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages for high-sensitivity proteomics applications.

• Polymeric monolithic stationary phases

• High-speed protein/peptide separations (< 10 min)

• High separation efficiency (> 200.000 plates/m)

• High column-to-column reproducibility

• High sensitivity in LC/MS

• Superior lifetime

• Available in 100 μm, 200 μm and 500 μm i.d 

• Available in 5 and 25 cm length (only 100 and 200 μm i.d.)

• nanoViper fitting

PepSwift Trap columns can be used for preconcentration and desalting of samples consisting of peptides and proteins without negative impact on the chromatographic performance or recovery of the compounds. Various ion-pairing agents can be used in the loading solvent and/or mobile phases to change the selectivity of the separation or improve the trapping efficiency.

*Tryptic digest of six proteins
**E. coli proteins
*** Dynamic loading capacity is defined as the 10% increase in peak width measured at half height induced by injected mass of a single peptide (tryptic peptide of cytochrome c for PepSwift RP) and intact protein (cytochrome c for ProSwift RP-10R), respectively

Separation of a peptide test mixture. Figure on separation on 1m long monolith.

Peak Width at Half Height (PWHH) for peptide separation.

Comparison of the influence of FA and TFA on separation of a peptide mixture.

Fast separation of tryptic peptides from 13 proteins. Peak capacities of up to 150 peaks in less than 15 min are routinely observed.

Ultra-high Resolution Separation of Tryptic Peptides on a 1m long PS-DVB Monolithic Column (4 × 25 cm)

Data Sheets
	PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Data Sheet

Application Notes
	AN 20972: Rapid Separation and Mass Spectrometry Detection of Intact Protein Samples Using Monolithic Capillary Columns
	AN 529: Capillary PS-DVB Monolithic Column of 500-μm i.d. for Peptide and Protein Separations in Top-Down Proteomics Studies
	AN 525: LC-MALDI MS Using Monolithic Capillary Columns

Technical Notes
	TN 106: Ultrahigh-Efficiency LC-MS/MS Separations of Proteolytic Digests on PepSwift Monolithic Columns

Manuals
	Thermo Scientific PepSwift and ProSwift Capillary Monolith Columns for Bioseparations Product Manual
	ProSwift RP-4H Capillary Quickstart